Functional Profiling

6. Functional Profiling#

6.1. Goal#

Quantify transcript abundance mapped to gene families or pathways.

6.2. Tools#

  • HUMAnN3 (with metatranscriptome support)

  • SAMSA2

  • Custom gene catalogs with featureCounts

6.3. Normalization#

Use CPM or TPM for expression; aggregate to pathways for biological interpretation.

6.4. Outputs#

  • pathway_abundance.tsv

  • gene_families.tsv

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